A young Boro side will head for Darlington on Tuesday (7.45pm).
Mark Tinkler will again dig deep into the Academy for the Under-21s friendly, but the squad will also feature a handful who have already experienced senior duty this summer.
Ajay Matthews, who scored at Hartlepool last weekend, will be joined by the likes of George Gitau, Jeremy Sivi, Daniel Nkrumah, and Fin Cartwright, who all featured in the weekend games.
Darlington warmed up against strong Harrogate Town side at the weekend and they will be boosted by the return of Jacob Hazel after international duty.